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E.coli Poly(A) Polymerase
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E. coli Poly (A) Polymerase (M0276) Vortex tube gently before use. Poly (A) Polymerase can be inactivated by adding EDTA at a final concentration of 10 mM to the reaction. Poly (A) Polymerase is compatible with the M-MuLV reverse transcriptase buffer. If the intention is to carry out the polyadenylation step followed by reverse transcription, we recommend to set-up these reactions in two ...
Polyadenylation of RNA molecules in bacteria and chloroplasts has been implicated as part of the RNA degradation pathway. The polyadenylation reaction is performed in Escherichia coli mainly by the enzyme poly(A) polymerase I (PAP I). In order to ...
Product description Poly(A) Polymerase (PAP) catalyzes the addition of adenosine to the 3′ end of RNA in a sequence-independent fashion. The polymerase is purified from an E. coli strain overexpressing E. coli Poly(A) Polymerase I, and supplied in 25 mM Tris (pH 8.0), 500 mM NaCl, 0.1 mM DTT, 1 mM MgCl2, 0.1 mM EDTA and 50% glycerol (v/v).
The Poly(A) Polymerase is intended for molecular biology applications. This product is neither intended for the diagnosis, prevention or treatment of a disease, nor has it been validated for such use either alone or in combination with other products. Need bulk, customized or optimized products for commercial purposes?
The polyadenylation reaction is performed in Escherichia coli mainly by the enzyme poly(A) polymerase I (PAP I). In order to understand the molecular mechanism of RNA poly-adenylation in bacteria, we characterized the biochemical properties of this reaction in vitro using the purified enzyme.
Poly (A) polymerase (PAP 1) from Escherichia coli is the primary enzyme responsible for synthesizing poly (A) tails on RNA molecules, signaling RNA degradation in bacterial cells. In vitro, PAP 1 is used to prepare libraries for RNAseq and to produce mRNA vaccines. However, E. coli PAP 1's toxicity and instability in low-salt buffers complicate its expression and purification. Here, we ...
Description This product is a GMP grade recombinant E. coli poly (a) polymerase, which can catalyze the incorporation of ATP into the 3' end of RNA in the form of AMP to form a poly (a) tail with 20-200 a bases. This product is a large-scale expression of recombinant poly (a) polymerase in E. coli, which conforms to GMP production and quality management specifications. All raw and auxiliary ...
Poly (A) Polymerase, E. coli. Poly (A) Polymerase catalyzes the addition of AMP to the 3' hydroxyl terminus of RNA. Poly (A) Polymerase can add poly (C) and poly (U) to RNA, but does so with much reduced efficiency.
Poly(A) polymerase I (PAP I) of Escherichia coli is a member of the nucleotidyltransferase (Ntr) super-family that includes the eukaryotic PAPs and all the known tRNA CCA-adding enzymes. Five highly con-served aspartic acids in the putative catalytic site of PAP I were changed to either alanine or proline, demonstrating their importance for polymerase activ-ity. A glycine that is absolutely ...
E. coli Poly (A) Polymerase does not depend on the presence of template, and can catalyze the sequential incorporation of ATP in the form of AMP into the 3´ terminal of RNA, i.e., the addition of polyA tail to the 3´ terminal of RNA. Poly (A) polymerase has A high tail adding efficiency and can add 20 to 200 A bases to the 3´ terminal of RNA. Polyadenylation improves the stability of RNA in ...