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E.coli Poly(A) Polymerase
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E. coli Poly (A) Polymerase (M0276) Vortex tube gently before use. Poly(A) Polymerase can be inactivated by adding EDTA at a final concentration of 10 mM to the reaction. Poly (A) Polymerase is compatible with the M-MuLV reverse transcriptase buffer. If the intention is to carry out the polyadenylation step followed by reverse transcription, we ...
How can the E. coli Poly(A) Polymerase be inactivated without heating up the reaction?; Does the E. coli Poly(A) Polymerase work in the M-MuLV reverse transcriptase buffer?; Can E. coli Poly(A) Polymerase be used to add a poly A tail to ssDNA?; Can E. coli Poly(A) Polymerase also add GTP, UTP and CTP to RNA?; Can I end-label a polyadenylated RNA molecule with a Cy3/5 or biotin-modified base ...
Abstract. Polyadenylation of RNA molecules in bacteria and chloroplasts has been implicated as part of the RNA degradation pathway. The polyadenylation reaction is performed in Escherichia coli mainly by the enzyme poly(A) polymerase I (PAP I). In order to understand the molecular mechanism of RNA polyadenylation in bacteria, we characterized the biochemical properties of this reaction in ...
The polymerase is purified from an E. coli strain overexpressing E. coli Poly(A) Polymerase I, and supplied in 25 mM Tris (pH 8.0), 500 mM NaCl, 0.1 mM DTT, 1 mM MgCl2, 0.1 mM EDTA and 50% glycerol (v/v). Using Poly(A) Polymerase Poly(A) Polymerase can be used to add a poly(A) tail to RNA transcripts. A poly(A) tail may increase mRNA stability ...
The polyadenylation reaction is performed in Escherichia coli mainly by the enzyme poly(A) polymerase I (PAP I). In order to understand the molecular mechanism of RNA poly-adenylation in bacteria, we characterized the biochemical properties of this reaction in vitro using the purified enzyme. Unlike the PAP from yeast nucleus, which is specific ...
A poly(A) polymerase was purified to homogeneity from extracts of Escherichia coli and subjected to N-terminal sequence analysis. The 25-residue amino acid sequence obtained was used to design primers for the amplification of the corresponding coding region by the PCR from an E. coli DNA template.
The gene encoding E. coli Poly(A) Polymerase expressed in E. coli. Supplied in: 25 mM Tris-HCl 500 mM NaCl 1 mM MgCl 2 0.1 mM DTT 0.1 mM EDTA 50% Glycerol pH 8.0 @ 25°C. Supplied with: 10x Poly(A) Polymerase Reaction Buffer. 10x Poly(A) Polymerase Reaction Buffer: 500 mM Tris-HCl 2.5 M NaCl 100 mM MgCl 2 pH 7.9 @ 25°C. Unit Definition:
Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins ... E.coli,whichisalsoamemberofthisfamily,hasextensive sequence homology with the N-terminal half of PAP I (Masters et al., 1990). The tRNA nucleotidyltransferase
E. coli Poly(A) Polymerase M0276 New England Biolabs. Your credentials are incorrect or you are trying to login with a non-existing webshop account. Our website shows product prices without login!Therefore webshop accounts that were only used to view prices have been deleted.
We have constructed a strain that overexpresses E coli poly(A) polymerase (PAP I). The recombinant protein was soluble, and a partially purified extract had high levels of poly(A) polymerising activity. An antiserum raised against the overexpressed PAP I has permitted two types of analysis: the identification of other E coli proteins that may ...