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Plasmid PxylA from Dr. Thorsten Mascher's lab contains the insert xylA promoter and is published in J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29. This plasmid is available through Addgene.
Promoter Pxyl together with promoter PlacI, repressor LacI (BBa_K1628201), promoter Pgrac (BBa_K1628202) and repressor XylR (BBa_K1628203) formed a metabolic toggle switch. We used this device to regulate the expression of odhAB genes in B. amyloliquefaciens NK-1 (showed in Figure 1).
XylR is a repressor of xylose operon regulating promoter Pxyl. XylR together with promoter PlacI, repressor LacI (BBa_K1628201), promoter Pxyl (BBa_K1628002) and promoter Pxyl (BBa_K1628002) formed a metabolic toggle switch. We used this device to regulate the expression of odhAB genes in Bacillus amyloliquefaciens NK-1 (showed in Figure 1).
pBAD-pXyl is a combined promoter of D-Xylose and L-arabinose sugar sensor systems which is designed to be activated only in the presence of both sugars in the medium therefore functioning as an AND logic gate. BIOLOGY. pXyl is an inducible promoter regulated by the transcriptional regulator XylR which, in Bacillus subtilis, regulates the ...
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Plasmid pXylA-agrCA-I from Dr. Cynthia Collins's lab contains the insert agrCA locus and is published in Biotechnol Bioeng. 2013 Nov;110(11):3003-12. doi: 10.1002/bit.24975. Epub 2013 Jul 5. This plasmid is available through Addgene.
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Description Promoter Xylose is an inducible promoter that has been designed for high expression in B.subtilis.Gene expression under the promoter xylose can be induced by addition of xylose.