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DEGs
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Learn how to find genes that show a difference in expression between sample groups using R packages edgeR and DESeq2. Follow the steps to pre-process, normalize, calculate fold-change and perform statistical tests on count data.
BACKGROUND The aim of this study was to investigate the differentially expressed genes (DEGs) relevant to prognosis of ovarian cancer by use of integrated bioinformatics analysis. MATERIAL AND METHODS The DEGs between normal ovariy tissue and ovarian cancer tissue were screened in GSE54388, GSE14407 …
発現変動遺伝子 (DEGs, Differentially Expressed Genes)とは、異なる条件やグループ間において発現量が大きく上昇または減少した遺伝子のことを言います。本ページでは発現変動遺伝子の抽出(DEG解析)について詳しく解説します。
Conclusions DEGs are frequently used to determine genotypical differences between two or more conditions of cells, in support of specific hypothesis-driven studies. Interpretation of this information can benefit significantly from the graphical representation of results files.
Identifying differentially expressed genes (DEGs) is a core task of transcriptome analysis, as DEGs can reveal the molecular mechanisms underlying biological processes. However, interpreting the biological significance of large DEG lists is challenging. Currently, gene ontology, pathway enrichment and protein-protein interaction analysis are common strategies employed by biologists ...
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This analysis showed that DEGs often reflect disease-induced changes in the transcriptome rather than disease-causing ones.
This article presents an extended review on the methods and softwares for identifying differentially expressed genes (DEGs) from RNA-Seq data, and a software tool that performs the analysis. The review evaluates six methods of mapping reads and nine methods of differential expression analysis, based on real RNA-Seq data and qRT-PCR data.
RNA sequencing (RNA-seq) is a powerful tool for studying gene expression, enabling researchers to identify differentially expressed genes (DEGs)—genes that are turned "on" or "off" under specific conditions, such as healthy versus diseased cells. Identifying DEGs helps scientists understand ...