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Documentation, product files, FAQs, and other support resources for the bcl2fastq and bcl2fastq2 Conversion Software
Learn how to install, run and configure bcl2fastq, a software tool for converting Illumina base call files to fastq format. See examples of sample sheets for dual index and single index libraries and tips for demultiplexing.
In addition to the command line options that control the behavior of BCL conversion, you can use the [Settings] section in the sample sheet configuration file to specify how the samples are processed. The following are the sample sheet settings for BCL conversion. Note that DRAGEN does not support the following sample sheet settings from bcl2fastq:
Running bcl2fastq Illumina bcl2fastq must be called with the correct --use-bases-mask argument, and other arguments, in order to properly demultiplex and output FASTQs for all the reads in a Chromium library.
Bcl2Fastq is a Linux-based software that converts base call files generated from an Illumina sequencing run to FASTQ files as well as demultiplex samples. This webinar focuses on Bcl2fastq v2+ and covers the following topics: requirements to run Bcl2Fastq; command line basics; commonly used options; and frequently asked 'How To….' questions.
The Illumina bcl2fastq Conversion Software demultiplexes sequencing data and converts BCL files into FASTQ files. bcl2fastq v1.8.4 is derived from CASAVA and it's fully compatible with the ...
Demultiplexing and FASTQ Generation Sequencing data stored in BCL format are processed through the bcl2fastq conversion software. The bcl2fastq conversion software demultiplexes data and converts BCL files to standard FASTQ file formats for downstream analysis. For each sequencing run, the VeriSeq NIPT Assay Software creates a sample sheet (SampleSheet.csv). This file contains sample ...
Learn how to use bcl2fastq to convert and demultiplex BCL basecall files from Illumina sequencing instruments into FASTQ files. The guide covers installation, input files, output folder, sample sheet, and adapter masking.
Learn how to use bcl2fastq to convert BCL basecall files to FASTQ files and demultiplex multiplexed samples from Illumina NextSeq 500 sequencing runs. This document covers installation, input files, output folder, and technical assistance.
bcl2fastq by Illumina. Contribute to savytskanatalia/bcl2fastq development by creating an account on GitHub.