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CRISPR-Cas13 systems use single-subunit RNA-guided Cas13 effectors for targeted RNA recognition and cleavage. This Review summarizes the recent advances in understanding the structural and ...
Pathogenic microorganisms have major impacts on human lives. Rapid and sensitive diagnostic tools are urgently needed to facilitate the early treatment of microbial infections and the effective control of microbial transmission. CRISPR-Cas13 employs ...
Figure 1. The Cas13 family. A. Four subtypes of CRISPR-Cas13 systems have been reported to date, which are classified based on the identity of the Cas13 protein and additional locus features. All known Cas13 family members contain two HEPN domains (shown in orange), which confer RNase activity. B. Cas13 can be reprogrammed to cleave a targeted ssRNA molecule through a short guide RNA with ...
An RNA-editing technology is developed to efficiently and specifically deplete and correct RNA directly in mammalian cells.
Despite ongoing efforts to study CRISPR systems, the evolutionary origins giving rise to reprogrammable RNA-guided mechanisms remain poorly understood. Here, we describe an integrated sequence/structure evolutionary tracing approach to identify the ancestors of the RNA-targeting CRISPR-Cas13 system. We find that Cas13 likely evolved from AbiF, which is encoded by an abortive infection-linked ...
CRISPR-Cas are adaptable natural prokaryotic defense systems that act against invading viruses and plasmids. Among the six currently known major CRISPR-Cas types, the type VI CRISPR-Cas13 is the only one known to exclusively bind and cleave foreign RNA. Within the last couple of years, this system h …
The class 2 type VI RNA-guided RNA-targeting CRISPR-Cas effector Cas13 can be engineered for RNA knockdown and binding, expanding the CRISPR toolset with a flexible platform for studying RNA in ...
Discover more about CRISPR-Cas13, including how it differs from CRISPR-Cas9 and what the technology can be used for.
Background CRISPR/Cas13 system, recognized for its compact size and specificity in targeting RNA, is currently employed for RNA degradation. However, the potential of various CRISPR/Cas13 subtypes, particularly concerning the knockdown of endogenous transcripts, remains to be comprehensively characterized in plants. Results Here we present a full spectrum of editing profiles for seven Cas13 ...
This study highlighted the latest progress in RNA editing targeted by the CRISPR-Cas13 family, and discussed the application of Cas13 in basic research, nucleic acid diagnosis, nucleic acid tracking, and genetic disease treatment.