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Cas6, a previously identified endoribonuclease present in a wide range of prokaryotes with the CRISPR-Cas system, binds and cleaves within the repeat sequences that separate the individual invader targeting elements in the CRISPR locus transcript.
Cas6 is an endoribonuclease that cleaves CRISPR RNAs within repeat sequences. (A) psiRNA biogenesis pathway model. The primary CRISPR transcript contains unique invader targeting or guide sequences (colored blocks) flanked by direct repeat sequences (R). Cas6 catalyzes site-specific cleavage within each repeat, releasing individual invader targeting units. The Cas6 cleavage products undergo ...
In Thermus thermophilus, a type III-associated Cas6 protein can cross-process type I-B pre-crRNAs and suppress the interference activity of the type I-B CRISPR-Cas system by competitively ...
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Cells often localize pathway enzymes in close proximity to reduce substrate loss via diffusion and to ensure that carbon flux is directed toward the desired product. To emulate this strategy for the biosynthesis of heterologous products in yeast, we have taken advantage of the highly specific Cas6-RNA interaction and the predictability of RNA hybridizations to demonstrate Cas6-mediated RNA ...
CRISPR-Cas systems provide bacteria and archaea with diverse mechanisms of adaptive immunity, illuminating the evolutionary pressures imposed by selfish genetic elements.
Cas6 endoribonuclease cleaves pre-CRISPR RNA (pre-crRNA) within the repeat region. Subsequently, the ribonucleoprotein (RNP) complex is assembled, while the 3′ end of crRNA is trimmed by an unknown nuclease.
Combining the RNA binding and processing capability of Cas6 proteins with toehold-mediated strand displacement enables the dynamic assembly and disassembly of metabolons to control enzyme function ...
Cas6 cleavage depends on the 5′ region required for binding, as well as sequences in the 3′ half of the CRISPR repeat (Carte et al. 2008). To investigate the molecular basis for Cas6 recognition of the CRISPR repeat RNA, we performed RNA footprinting with recombinant P. furiosus Cas6 protein and radiolabeled CRISPR repeat RNA (Fig. 2).
Identification of miniaturized type I-F2 CRISPR-Cas systems Type I-F2 systems consist of five Cas proteins: Cas1, Cas3, Cas5, Cas6, and Cas7, without the large and small subunits (Fig. 1a) 30, 31 ...