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dh10b
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DH10B is a high efficiency, chemically competent E. coli strain for cloning DNA with methylated bases. It has blue/white screening, outgrowth medium, and is suitable for cDNA libraries and site directed mutagenesis.
NEB 10-beta Competent E. coli is a derivative of the popular DH10B. It is T1 phage resistant and endonuclease I (endA1) deficient for high- quality plasmid preparations. High efficiency strain ideal for cloning large plasmids and BACs Available in single-use vials, 200 μl vials, 96-well plate and electrocompetent format No dry ice surcharge on competent cell shipments Outgrowth medium ...
Escherichia coli DH10B was designed for the propagation of large insert DNA library clones. It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids. The strain was ...
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
Learn how to transform DH10B Competent Cells, a product for cloning DNA containing methylcytosine and methyladenine. Find out the genotype, contents, storage, and transformation efficiency of these cells.
The DH10B competent E. coli has a transformation efficiency of 1 x 10 9 cfu/µg for supercoiled DNA and is ideal for high-efficiency cloning and plasmid propagation. It allows stable replication of high-copy number plasmids. In comparison with DH5a, methylated DNA from genomic preparation could be efficiently transformed into DH10B, therefor, the DH10B cells are more applicable than DH5a in ...
MegaX DH10B T1 R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 10 10 cfu/μg of pUC DNA, making MegaX DH10B T1 R cells ideal for highly demanding cloning or cDNA and gDNA library construction applications.
The DH10B chemically competent E. coli cells are suitable for cloning and propagation of large size plasmids (10-150 kb in size). DH10B strain is known for stable replication of high-copy number plasmids and demonstrates improved cloning efficiency of mammalian DNA containing methylated cytosine or adenine.
Intact Genomics ig® 10B chemically competent cells (E. coli) are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.
NEB offers a range of competent cells for cloning and protein expression, including DH10B, a derivative of DH10B with high transformation efficiency and blue/white screening. Learn about the features, advantages and formats of NEB competent cells and compare them with other strains.