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13C metabolic flux analysis-guided metabolic engineering of Escherichia ...

Flux distributions in acetol-producing and non-producing E. coli strains HJ06 (a), HJ06C (b), HJ06N (c), HJ06P (d), and HJ06PN (e). The flux values of the best fit were normalized to the specific glycerol consumption rate of 100 Metabolism-wide NADPH production and consumption in HJ06 and HJ06C were then estimated based on metabolic fluxes (Fig ...

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Flux distributions in acetol-producing and non-producing E. coli...

Download scientific diagram | Flux distributions in acetol-producing and non-producing E. coli strains HJ06 (a), HJ06C (b), HJ06N (c), HJ06P (d), and HJ06PN (e). The flux values of the best fit ...

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13C metabolic flux analysis-guided metabolic engineering of Escherichia ...

Valorization of glycerol, the main by-product of biodiesel, has contributed to the viability of biofuel economy. We performed 13C-MFA and measured intracellular pyridine nucleotide pools in a first-generation acetol producer strain (HJ06) and a non-producer strain (HJ06C), and identified that engineering the NADPH regeneration is a promising ...

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13C metabolic flux analysis-guided metabolic engineering of ... - PubMed

Results: We performed 13 C-MFA and measured intracellular pyridine nucleotide pools in a first-generation acetol producer strain (HJ06) and a non-producer strain (HJ06C), and identified that engineering the NADPH regeneration is a promising target.

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