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Learn how to use Hoechst 33342, a cell-permeant nuclear counterstain that emits blue fluorescence, for fluorescence microscopy. Find out how to prepare the dye stock solution, label cells, and image them with the standard filter set.
Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. [1][2] These bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33,342nd compound made by the company.
Hoechst AG was founded in 1863 and became part of IG Farben during World War I. It merged with Rhône-Poulenc in 1999 to form Aventis, and later with Sanofi in 2005.
DAPI and Hoechst are popular nuclear counterstains for use in all fluorescent techniques. The blue fluorescence is vivid contrast to green, yellow, red, and far red fluorescent probes and labels.
Performing nuclear staining with DAPI or Hoechst? Check out Biotium's detailed Hoechst & DAPI staining protocols for staining live cells or fixed cells & tissues.
Höchst (German pronunciation: [ˈhøːçst] ⓘ) [2] is a neighbourhood and market town in the Ortsbezirk of Frankfurt-West in Frankfurt am Main, Germany. Höchst is situated 10 kilometres (6.2 mi) west of Frankfurt city centre, on the north bank of the Main at the confluence with the River Nidda. Its old town is famous for around 400 timber framed houses. [3][4]
Learn about the origins, development, and diversification of Hoechst A.G., the world's largest chemical manufacturer. From dyestuffs to pharmaceuticals, from IG Farben to global network, discover the company's milestones and achievements.
Explore the principles of Hoechst staining, its binding mechanisms, dye variations, and applications in fluorescence imaging for live and fixed cell studies.
Hoechst Structure The chemical structure of Hoechst dyes is based on a benzimidazole ring, comprising two benzene rings and one imidazole ring. Its molecular formula is C₂₇H₂₆N₂O, with a molecular weight of approximately 378.51 g/mol. The primary difference between Hoechst 33258 and Hoechst 33342 lies in the substituents on their side chains. Hoechst 33258 has a methyl substituent ...
Hoechst stains are part of a family of blue fluorescent dyes commonly used to stain DNA. It gets incorporated biosynthetically into interphase nuclei in fixed cytologic preparations and in unfixed cultured cells. [1] It can also be used to detect biosynthetic incorporation of 5-bromodeoxyuridine into the DNA of living cells. Hoechst stains property may allow fluorescence-activated cell and ...