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iTRAQ is a technique to quantify proteins from different sources in a single experiment using stable isotope tags. Learn about the procedure, data evaluation and software tools for iTRAQ analysis.
iTRAQ is a technique that uses isobaric tags to multiplex and quantify up to eight protein samples by MS/MS. Learn about the sample preparation, analysis, and quantitation methods for iTRAQ at Yale Keck Proteomics.
The iTRAQ technology uses four or eight distinct isobaric tags containing the same total mass, which allows multiplexing of samples, thereby significantly increasing throughput and minimizing experimental variability. Upon fragmentation during tandem mass spectrometry, the tags release reporter ions of different masses that provide a relative ...
A novel, MS-based approach for the relative quantification of proteins, relying on the derivatization of primary amino groups in intact proteins using isobaric tag for relative and absolute quantitation (iTRAQ) is presented. Due to the isobaric mass design of the iTRAQ reagents, differentially label …
iTRAQ is a multiplexed isobaric tagging technique for relative quantification of proteins by MS/MS. This article surveys the iTRAQ literature and discusses its strengths and weaknesses, as well as its comparison with other quantitative proteomic methods.
The TMT tag (Pierce) is similar to iTRAQ but with amine, cysteine or carbonyl reactive tags, a mass reporter region (6 different tags with masses 126 to 131) and a mass "normalizer" region. For the amine reactive tag, the normalizer region keeps the tag at a constant mass of 229. Hence, using these tags, 2- 8 plex multiplexed experiments ...
iTRAQ® is a powerful quantitative proteomics technique for broad and unbiased quantification of proteins expressed across multiple biological samples. iTRAQ generates relative quantitative proteome profiles and enables you to perform comparisons across multiple samples in a single experiment.
Also, iTRAQ can reduce overall time and variation. On the other hand, iTRAQ reagents are extremely costly and also extremely sensitive to contamination from salts. Moreover, sophisticated software is required for analyzing iTRAQ data. Another disadvantage is the variability arising due to the inefficient enzymatic digestion. An example of iTRAQ
But since iTRAQ data are obtained by running the isolated peptides through MS/MS, this probability model provides an alternative way of studying the missingness in iTRAQ. Wang et al. [ 31 ] proposed to first remove sources of systamatic variation between MS profiles via global normalization, and then to investigate the intensity-dependent ...
Relative Quantification: iTRAQ™ iTRAQ™ is a multiplexed protein quantitation strategy that provides relative measurements of proteins in complex mixtures. For quantitative protein analysis the isobaric mass labels are covalently attached at the N-termini and lysine side chains of peptides in a digest mixture.