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LaG16 and GFP-enhancer can bind to GFPuv at the same time. To confirm that LaG16 and GFP-enhancer can bind to GFPuv noncompetitively in vitro, we used the FSEC method 22.After the addition of only ...
The LaG16 binding site was on the opposite side of the GFP β-barrel from the binding site of the GFP-enhancer, another anti-GFP nanobody, indicating that the GFP-enhancer and LaG16 can bind to GFP together. Thus, we further designed 3 linkers of different lengths to fuse LaG16 and GFP-enhancer together, and the GFP binding of the three ...
Plasmid N8his-GFPenhancer-GGGGS4-LaG16 from Dr. Motoyuki Hattori's lab contains the insert GFPenhancer-GGGGS4-LaG16 and is published in Sci Rep. 2020 Apr 10;10(1):6239. doi: 10.1038/s41598-020-62606-7. This plasmid is available through Addgene.
The LaG16 binding site was on the opposite side of the GFP β-barrel from the binding site of the GFP-enhancer, another anti-GFP nanobody, indicating that the GFP-enhancer and LaG16 can bind to GFP together. Thus, we further designed 3 linkers of different lengths to fuse LaG16 and GFP-enhancer together, and the GFP binding of the three ...
In CDR3 (Fig. 1D), Arg102 of LaG16 and Glu90 of GFPuv form a salt bridge, resulting in the high affinity of LaG16 to GFPuv. In addition, hydrophobic interactions are shown between Val105 of LaG16 ...
LAG16 and LAG30 have 90% sequence identity (Supplementary Fig. 5) and bind GFP at closely located epitopes on the opposite to the chromophore hydroxyl side of the protein β-barrel 35,36 (Fig. 1d).
LaG16. Part:BBa_K2549003. Designed by: Rongrong Du Group: iGEM18_Fudan (2018-10-01) anti-GFP (LaG16) Anti-GFP (LaG16) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP. It was used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP.
Lentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic Notch Depositing Lab. Wendell Lim. Publication. Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi: 10.1016/j.cell.2016.09.011. Epub 2016 Sep 29. ( How to cite ) Sequence Information ...
The LaG16 binding site was on the opposite side of the GFP β-barrel from the binding site of the GFP-enhancer, another anti-GFP nanobody, indicating that the GFP-enhancer and LaG16 can bind to GFP together. Thus, we further designed 3 linkers of different lengths to fuse LaG16 and GFP-enhancer together, and the GFP binding of the three ...
LaG16: B. 147: Lama glama: Mutation(s): 0 : Entity Groups Sequence Clusters: 30% Identity 50% Identity 70% Identity 90% Identity 95% Identity 100% Identity: Sequence Annotations Expand: Reference Sequence; Small Molecules. Modified Residues 1 Unique; ID Chains Type Formula 2D Diagram Parent; CRO Query on CRO: A.