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raw264.7和thp 1
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Human THP-1 and mouse RAW264.7 cell line models have been widely used in various macrophage-associated studies, while the similarities and differences in protein expression profiles between the two macrophage models are still largely unclear. In this study, the protein expression profiles of M1 and M2 phenotypes from both THP-1 and RAW264.7 ...
因此借助raw264.7的可诱导分化能力，加深对破骨细胞的研究，能够帮助我们更好的对相关疾病进行预防和治疗。 thp-1细胞. 在科学研究中，thp-1细胞常被诱导分化为m1和m2型巨噬细胞和泡沫细胞，用于炎症的研究。
Figure 1 Workflow of the study and validation of polarization models.(A) Workflow of this study about quantitative proteome analysis of three types of polarized macrophages from human THP-1 and mouse RAW264.7 cells.(B, C) Measurement of known markers for M1/M2 phenotypes in polarized human THP-1 macrophages (B) and mouse RAW264.7 macrophages (C) using the immunofluorescence staining method.
Human THP-1 and mouse RAW264.7 cell line models have been widely used in various macrophage-associated studies, while the similarities and differences in protein expression profiles between the ...
THP-1's are a human monocyte-like cell line. They can be differentiated into a cell similar to a macrophage although there are significant phenotypic variations including poor (compared to real ...
cells: RAW264.7 cells (5*105 cells/ml),100 µl/well) and PMA-treated THP-1 (10 5 cells/ml) were stimulated with the indicated concentrations from LPS. The cell culture medium was collected after ...
thp-1细胞为悬浮细胞，相比于ml-2、hl-60、u937等白血病细胞系，thp-1细胞具有更接近于人原代单核细胞的形态和功能特征（包括细胞分化标记）。 并且相对于人外周血单核细胞（PBMC），THP-1更易在实验室中培养和扩增，且具有更稳定的基因背景，不存在PBMC的个体 ...
相对于人外周血单核细胞（pbmc），thp-1更易在实验室中培养和扩增，且具有更稳定的基因背景，不存在pbmc的个体差异性问题，利于实验结果的重现。 RAW264.7细胞是小鼠单核巨噬细胞白血病细胞，为贴壁细胞，是从用Abelson鼠科白血病病毒诱导的雄性小鼠的肿瘤中 ...
Differences in these over-expressed proteins between polarized THP-1 and RAW264.7 macrophages may be due to the differences of the two cell lines response to the same stimulus. To a certain extent, this study illustrated the differences of protein expression between human THP-1 and mouse RAW264.7 polarized macrophages, and it should be taken ...
thp-1细胞为悬浮细胞，相比于ml-2、hl-60、u937等白血病细胞系，thp-1细胞具有更接近于人原代单核细胞的形态和功能特征（包括细胞分化标记）。 并且相对于人外周血单核细胞（PBMC），THP-1更易在实验室中培养和扩增，且具有更稳定的基因背景，不存在PBMC的